概述

  • 产品名称Anti-SRPR beta抗体
    参阅全部 SRPR beta 一抗
  • 描述
    兔多克隆抗体to SRPR beta
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 181-230 (QDIAMAKSAK LIQQQLEKEL NTLRVTRSAA PSTLYSSSTA PAQLGKKGKE) of Human SRPR beta (NP_067026).

  • 阳性对照
    • Hela cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab81324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

IHC-P 1/100.

靶标

  • 功能Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. Has GTPase activity. May mediate the membrane association of SRPR.
  • 序列相似性Belongs to the SRP receptor beta subunit family.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APMCF1 antibody
    • Protein APMCF1 antibody
    • Signal recognition particle receptor B subunit antibody
    • Signal recognition particle receptor beta subunit antibody
    • Signal recognition particle receptor subunit beta antibody
    • SR-beta antibody
    • SRbeta antibody
    • SRPR beta antibody
    • Srprb antibody
    • SRPRB_HUMAN antibody
    see all

Anti-SRPR beta antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling SRPR beta with ab81324 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SRPR beta. Right - Merge.


  • Predicted band size : 30 kDa
    Western blot analysis of Jurkat cell lysates labeling SRPR beta with ab81324 at 1.0µg/ml


  • Predicted band size : 30 kDa
    Western blot analysis of 721_B cell lysates labeling SRPR beta with ab81324 at 1.0µg/ml
  • Anti-SRPR beta antibody (ab81324) at 1 µg/ml + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-SRPR beta antibody (ab81324)参考文献

ab81324 has not yet been referenced specifically in any publications.

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