存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
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纯度 Protein G purified
纯化说明 ab71675 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).
功能 May be involved in proliferation or differentiation of keratinocytes in the skin.
序列相似性 Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Information by UniProt
EC 220.127.116.11 antibody
Anti-SRMS antibody - N-terminal 图像
Western blot - SRMS antibody - N-terminal (ab71675)
Anti-SRMS antibody - N-terminal (ab71675) at 1/100 dilution + HL-60 cell lysate at 12.5 µg Secondary HRP-anti-rabbit Predicted band size : 55 kDa Observed band size : 65 kDa ( why is the actual band size different from the predicted?) Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SRMS antibody - N-terminal (ab71675)
ab71675 at 1/50 dilution, staining SRMS in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
Anti-SRMS antibody - N-terminal (ab71675)参考文献
has not yet been referenced specifically in any publications.
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