兔多克隆抗体to SRMS - N-terminal
Synthetic peptide from the N-terminal region of human SRMS conjugated to KLH
HL-60 cell lysate. Human breast carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71675 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).
May be involved in proliferation or differentiation of keratinocytes in the skin.
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Information by UniProt
EC 18.104.22.168 antibody
Western blot - SRMS antibody - N-terminal (ab71675)
Anti-SRMS antibody - N-terminal (ab71675) at 1/100 dilution + HL-60 cell lysate at 12.5 µg
Predicted band size:
Observed band size:
65 kDa (
why is the actual band size different from the predicted?
Additional bands at:
30 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SRMS antibody - N-terminal (ab71675)
ab71675 at 1/50 dilution, staining SRMS in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
has not yet been referenced specifically in any publications.
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