重组Anti-srGAP2抗体[EP2506(2)] (ab124958)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2506(2)] to srGAP2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-srGAP2抗体[EP2506(2)]
参阅全部 srGAP2 一抗 -
描述
兔单克隆抗体[EP2506(2)] to srGAP2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human srGAP2 aa 1000-1100. The exact sequence is proprietary.
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阳性对照
- WB: HEK-293, and Mouse and Rat brain lysate.
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常规说明
This product has switched from a hybridoma to recombinant production method on 7th September 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2506(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124958于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 121 kDa). |
靶标
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功能
Putative GTPase-activating protein for Rho family small GTPases. -
组织特异性
Expression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested. -
序列相似性
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 23380 Human
- Entrez Gene: 14270 Mouse
- Entrez Gene: 360840 Rat
- Omim: 606524 Human
- SwissProt: O75044 Human
- SwissProt: Q91Z67 Mouse
- SwissProt: D4A208 Rat
- Unigene: 497575 Human
see all -
别名
- ARHGAP34 antibody
- FBP2 antibody
- FNBP2 antibody
see all
图片
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Anti-srGAP2 antibody [EP2506(2)] (ab124958) at 1/1000 dilution + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-srGAP2 antibody [EP2506(2)] (ab124958) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124958 被引用在 2 文献中.
- Mondal C et al. A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling. Cell Rep 40:111358 (2022). PubMed: 36130489
- Sporny M et al. Structural History of Human SRGAP2 Proteins. Mol Biol Evol 34:1463-1478 (2017). PubMed: 28333212