Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-srGAP2 antibody (ab121977)This image is courtesy of Alain Chedotal (Institut Vision, France)
All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilution
Lane 1 : COS7 Whole cell lysate Lane 2 : HEK293H Whole Cell Lysate Lane 3 : HEK293H Over expressing human srGAP1 Lane 4 : HEK293H Over expressing human srGAP2 Lane 5 : HEK293H Over expressing human srGAP3 Lane 6 : HeLa cells over expressing mouse srGAP2 Lane 7 : Mouse E14 hindbrain lysate
Lysates/proteins at 30 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution