Anti-srGAP2抗体(ab121977)

概述

  • 产品名称Anti-srGAP2抗体
    参阅全部 srGAP2 一抗
  • 描述
    兔多克隆抗体to srGAP2
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    antigen: LEPLKTSPVV APTSEPSSPL HTQLLKDPEP AFQRSASTAG DIACAFRPVK SVKMAAPVKP PATRPKPTVF PKTNATSPGV NSSTSPQST, corresponding to amino acids 977-1065 of Human srGAP2.

  • 阳性对照
    • Human adrenal gland tissue.
  • 常规说明


    ab121977 is mono-specific.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab121977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100. Detects a band of approximately 170 kDa (predicted molecular weight: 121 kDa).

Abcam recommends 5% milk blocking

ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 功能Putative GTPase-activating protein for Rho family small GTPases.
  • 组织特异性Expression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested.
  • 序列相似性Contains 1 FCH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHGAP34 antibody
    • FBP2 antibody
    • FNBP2 antibody
    • FNBP2_HUMAN antibody
    • Formin binding protein 2 antibody
    • Formin-binding protein 2 antibody
    • Rho GTPase-activating protein 34 antibody
    • SLIT ROBO Rho GTPase activating protein 2 antibody
    • SLIT-ROBO Rho GTPase-activating protein 2 antibody
    • srGAP2 antibody
    • SRGAP3 antibody
    see all

Anti-srGAP2 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilution

    Lane 1 : COS7 Whole cell lysate
    Lane 2 : HEK293H Whole Cell Lysate
    Lane 3 : HEK293H Over expressing human srGAP1
    Lane 4 : HEK293H Over expressing human srGAP2
    Lane 5 : HEK293H Over expressing human srGAP3
    Lane 6 : HeLa cells over expressing mouse srGAP2
    Lane 7 : Mouse E14 hindbrain lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 121 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa (possible tagged protein).

    Exposure time : 5 minutes

    This image is courtesy of Alain Chedotal (Institut Vision, France)

    Blocking: 5% MIlk

  • ab121977, at 1/10 dilution, staining srGAP2 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.

Anti-srGAP2 antibody (ab121977)参考文献

ab121977 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step 0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0,25%TritonX100
Fixative Paraformaldehyde
Username

Dr. Alain Chedotal

Verified customer

提交于 May 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
Loading amount 30 µg
Specification HEK293H, COS7, mouse hindbrain lysate E14
Gel Running Conditions Reduced Denaturing (4-15% SDS gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Alain Chedotal

Verified customer

提交于 Apr 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"