概述

  • 产品名称Anti-SRGAP1抗体
    参阅全部 SRGAP1 一抗
  • 描述
    小鼠单克隆抗体to SRGAP1
  • 特异性Customer reported cross-reactivity with SRGAP2 and SRGAP3. Please see the Abreview for transfected Cos cells.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: PLDPETIAQD IEETMNTALN ELRELERQST AKHAPDVVLD TLEQVKNSPT PATSTESLSP LHNVALRSSE PQIRRSTSSS SDTMSTFKPM VAPRMGVQL, corresponding to amino acids 952-1051 of Human SRGAP1

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab57504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
  • 组织特异性Expressed in brain, lung, kidney, and testis.
  • 序列相似性Contains 1 FCH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.
  • 结构域The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4930572H05Rik antibody
    • ARHGAP13 antibody
    • FLJ22166 antibody
    • KIAA1304 antibody
    • RGD1566260 antibody
    • Rho GTPase activating protein 13 antibody
    • Rho GTPase-activating protein 13 antibody
    • SLIT ROBO Rho GTPase activating protein 1 antibody
    • SLIT-ROBO Rho GTPase-activating protein 1 antibody
    • SRGAP 1 antibody
    • srGAP1 antibody
    • SRGP1_HUMAN antibody
    see all

Anti-SRGAP1 antibody 图像

  • ICC/IF image of ab57504 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab57504 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • SRGAP1 antibody (ab57504) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab57504 staining SRGAP1 in Human Fetal Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with 0.1% Triton-X-100 i PBS; antigen retrieval was by heat mediation with a citrate buffer (10mM). Samples were incubated with primary antibody (1/900 in 3% serum/0.1% Triton-X-100 in PBS) for 16 hours at 4°C. A Biotin-conjugated Horse anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab57504 staining SRGAP1 in human fetal brainstem tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton X-100/PBS. Samples were then incubated with 3% serum and the primary antibody at a 1/1000 dilution, for 16 hours at 4°C. A biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody at a 1/200 dilution.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab57504 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57504, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-SRGAP1 antibody (ab57504)参考文献

This product has been referenced in:
  • Chen ZB  et al. Slit-Robo GTPase-Activating Proteins are Differentially Expressed in Murine Dorsal Root Ganglia: Modulation by Peripheral Nerve Injury. Anat Rec (Hoboken) 295:652-60 (2012). Mouse . Read more (PubMed: 22271578) »
  • Ip BK  et al. The corticofugal neuron-associated genes ROBO1, SRGAP1, and CTIP2 exhibit an anterior to posterior gradient of expression in early fetal human neocortex development. Cereb Cortex 21:1395-407 (2011). IHC ; Human . Read more (PubMed: 21060114) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Immunohistochemistry (Frozen sections)
Blocking step 0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0,25%TritonX100
Fixative Paraformaldehyde
Username

Dr. Alain Chedotal

Verified customer

提交于 May 17 2013

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
Loading amount 30 µg
Specification HEK293H, COS7, mouse hindbrain lysate E14
Gel Running Conditions Reduced Denaturing (4-15% SDS gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Alain Chedotal

Verified customer

提交于 Apr 11 2013

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Human Fetal Brainstem)
Specification Human Fetal Brainstem
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X-100 / PBS
Username

Abcam user community

Verified customer

提交于 Oct 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Fetal Brain)
Specification Human Fetal Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate Buffer
Permeabilization Yes - 0.1% Triton-X-1000 in PBS
Username

Abcam user community

Verified customer

提交于 Oct 04 2010

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (COS cell with transfected cDNA)
Loading amount 15 µg
Specification COS cell with transfected cDNA
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"