概述

  • 产品名称
  • 描述
    兔多克隆抗体to SREBP1
  • 特异性
    ab28481 recognises SREBP 1. B
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    MLQLINNQDSDFPGLF

    , corresponding to amino acids 32-47 of Mouse SREBP 1

  • 阳性对照
    • Mouse and rat liver. Rat kidney.

应用

Our Abpromise guarantee covers the use of ab28481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000. Can be blocked with Mouse SREBP1 peptide (ab31099).

Detects an ~68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominant band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished.

ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/2000.

靶标

  • 功能
    Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
  • 组织特异性
    Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
  • 序列相似性
    Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻译后修饰
    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
    Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
  • 细胞定位
    Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADD 1 antibody
    • bHLHd1 antibody
    • Class D basic helix-loop-helix protein 1 antibody
    • D630008H06 antibody
    • Processed sterol regulatory element-binding protein 1 antibody
    • SRBP1_HUMAN antibody
    • SREBF 1 antibody
    • SREBF1 antibody
    • SREBP 1 antibody
    • SREBP 1c antibody
    • SREBP-1 antibody
    • SREBP1 antibody
    • Sterol regulatory element binding protein 1 antibody
    • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
    • Sterol regulatory element binding transcription factor 1 antibody
    • Sterol regulatory element-binding transcription factor 1 antibody
    see all

Anti-SREBP1 antibody 图像

  • All lanes : Anti-SREBP1 antibody (ab28481) at 1/1000 dilution

    Lane 1 : Mouse Liver Whole Cell Lysate
    Lane 2 : Rat Liver Whole Cell Lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-Conjugate

    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemical analysis of formalin-fixed C2C12 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification was 60X
    The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains.

  • ab28481 staining SREBP1 in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 15 minutes at 25°C. Samples were incubated with primary antibody (1/2000 in goat serum) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

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  • Immunocytochemical analysis of formalin-fixed HepG2 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification was 60X
    The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains.

  • Immunocytochemical analysis of formalin-fixed NIH 3T3 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification is 60X
    The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains.

  • ICC/IF image of ab28481 stained HepG2 cells. The cells were 10% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28481, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, goat anti rabbit DyLight® 488 IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Frozen sections) analysis of mouse liver tissue labeling SREBP1 with ab28481 at 1/200 dilution. Tissue was fixed in acetone followed by blocking with 10% serum for 1 hour at 21°C. The tissue was stained with ab28481 at 1/200 for 12 hours in PBS+0,1% Triton X at 4°C. A polyclonal goat anti-rabbit Alexa Fluor® 594 secondary antibody was used at 1/1000 dilution.

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  • Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling SREBP1 with ab28481 at 1/200 dilution. Tissue was fixed in acetone followed by blocking with 10% serum for 1 hour at 21°C. The tissue was stained with ab28481 at 1/200 for 12 hours in PBS+0,1% Triton X at 4°C. A polyclonal goat anti-rabbit Alexa Fluor® 594 secondary antibody was used at 1/1000 dilution.

    See Abreview

  • Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling SREBP1 with ab28481 at 1/200 dilution. Tissue was fixed in acetone followed by blocking with 10% serum for 1 hour at 21°C. The tissue was stained with ab28481 at 1/200 for 14 hours in PBS+0,1% Triton X at 4°C. A polyclonal goat anti-rabbit Alexa Fluor® 594 secondary antibody was used at 1/1000 dilution.

    See Abreview

Anti-SREBP1 antibody (ab28481)参考文献

This product has been referenced in:
  • Ren T  et al. The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-a. Nutrients 9:N/A (2017). Read more (PubMed: 28264426) »
  • Zhang Y  et al. Effect of Tetramethylpyrazine on Atherosclerosis and SCAP/SREBP-1c Signaling Pathway in ApoE(-/-) Mice Fed with a High-Fat Diet. Evid Based Complement Alternat Med 2017:3121989 (2017). WB ; Mouse . Read more (PubMed: 28491104) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue lysate)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
25 µg
Specification
Rat liver tissue lysate
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Aug 23 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (rat liver tissue lysate)
Loading amount
140 µg
Specification
rat liver tissue lysate
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Nov 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (liver)
Specification
liver
Fixative
Acetone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2)
Specification
HepG2
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2012

Thank you for your reply.
The immunogen for ab28481 is conserved in all isoforms of SREBP1, and it should detect SREBP1.C. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us.

I am assuming customer is interested in molecular weight of proteins these antibodies detect. The predicted band size is as follows;

ab28481; the molecular weight of bands would be 121, 113, 111 and 124 kD...

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Loading amount
30 µg
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3
Username

Abcam user community

Verified customer

提交于 Jun 18 2007

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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