概述

  • 产品名称Anti-SRD5A2抗体
    参阅全部 SRD5A2 一抗
  • 描述
    山羊多克隆抗体to SRD5A2
  • 特异性ab27469 recognises SRD5A2.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    QVQCQQSPVLAG

    , corresponding to N terminal amino acids 2-13 of Human SRD5A2

  • 阳性对照
    • Human testes or prostate lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab27469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).Can be blocked with SRD5A2 peptide (2-13) (ab45681).
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • 组织特异性Expressed in high levels in the prostate and many other androgen-sensitive tissues.
  • 疾病相关Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
  • 序列相似性Belongs to the steroid 5-alpha reductase family.
  • 细胞定位Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
    • 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
    • 5 alpha SR2 antibody
    • 5 alpha-SR2 antibody
    • 5ART2 antibody
    • e II 5 alpha reductase antibody
    • EC antibody
    • MGC138457 antibody
    • Micropenis, included antibody
    • S5A2_HUMAN antibody
    • S5AR 2 antibody
    • SR type 2 antibody
    • SRD5A2 antibody
    • Steroid 5 alpha reductase 2 antibody
    • Steroid 5-alpha-reductase 2 antibody
    • Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) antibody
    • Type II 5-alpha reductase antibody
    see all

Anti-SRD5A2 antibody 图像

Anti-SRD5A2 antibody (ab27469)参考文献

This product has been referenced in:
  • Kelleher MA  et al. Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig. Reprod Sci 20:1365-75 (2013). Read more (PubMed: 23585339) »
  • Kelleher MA  et al. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. J Endocrinol 208:301-9 (2011). Read more (PubMed: 21149437) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate LNCaP cells)
Specification Prostate LNCaP cells
Fixative Paraformaldehyde
Permeabilization Yes - spot 0.2% Triton-X
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C
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提交于 Aug 17 2012

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