The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
ab31295 at 4-6 µg/ml, staining SRD5A1 in Human Prostate tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Image shows reticulate staining in the cytoplasm of secretory cells in the gland. Steamed antigen retrieval with pH 6 citrate buffer was performed, and HRP-staining was used.