概述

  • 产品名称
    Anti-SRC3抗体
    参阅全部 SRC3 一抗
  • 描述
    兔多克隆抗体to SRC3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Horse, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 576-625 (DQNPVESSMC QSNSRDHLSD KESKESSVEG AENQRGPLES KGHKKLLQLL) of human KAT13B/ AIB1 (NP_006525).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • 293T cell lysate

应用

Our Abpromise guarantee covers the use of ab85452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 155 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
  • 组织特异性
    Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • 序列相似性
    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域
    Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
  • 翻译后修饰
    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.
  • 细胞定位
    Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • ACTR antibody
      • AIB 1 antibody
      • AIB-1 antibody
      • AIB1 antibody
      • Amplified in breast cancer 1 antibody
      • Amplified in breast cancer 1 protein antibody
      • bHLHe42 antibody
      • CAGH 16 antibody
      • CAGH16 antibody
      • CBP interacting protein antibody
      • CBP-interacting protein antibody
      • Class E basic helix-loop-helix protein 42 antibody
      • CTG 26 antibody
      • CTG26 antibody
      • KAT13B / AIB1 antibody
      • MGC141848 antibody
      • NCOA 3 antibody
      • NCoA-3 antibody
      • Ncoa3 antibody
      • NCOA3_HUMAN antibody
      • Nuclear receptor coactivator 3 antibody
      • pCIP antibody
      • RAC 3 antibody
      • RAC-3 antibody
      • RAC3 antibody
      • Receptor associated coactivator 3 antibody
      • Receptor-associated coactivator 3 antibody
      • SRC 3 antibody
      • SRC-3 antibody
      • SRC3 antibody
      • Steroid receptor coactivator protein 3 antibody
      • Thyroid hormone receptor activator molecule 1 antibody
      • TNRC14 antibody
      • TNRC16 antibody
      • TRAM 1 antibody
      • TRAM-1 antibody
      • TRAM1 antibody
      see all

    图片

    • Western blot - Anti-SRC3 antibody (ab85452)
      Western blot - Anti-SRC3 antibody (ab85452)
      Western blot analysis of MCF7 cell lysate labeling SRC3 with ab85452 at 1.0µg/ml.
    • Western blot - KAT13B / AIB1 antibody (ab85452)
      Western blot - KAT13B / AIB1 antibody (ab85452)
      Anti-SRC3 antibody (ab85452) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 155 kDa
      Observed band size: 155 kDa



      Gel concentration: P6-18%
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SRC3 antibody(ab85452)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SRC3 antibody(ab85452)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SRC3 with ab85452 at 5.0µg/ml.

    文献

    ab85452 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab85452？请让我们知道，以便我们可以引用本数据表中的参考文章。

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