概述
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产品名称Anti-SRC3抗体
参阅全部 SRC3 一抗 -
描述兔多克隆抗体to SRC3
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经测试应用适用于: IHC-P, WBmore details
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种属反应性与反应: Human
预测可用于: Rat, Horse, Cat, Dog, Pig
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免疫原
Synthetic peptide corresponding to a region within the internal sequence amino acids 576-625 (DQNPVESSMC QSNSRDHLSD KESKESSVEG AENQRGPLES KGHKKLLQLL) of human KAT13B/ AIB1 (NP_006525).
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阳性对照
- 293T cell lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab85452 is purified by peptide affinity chromatography method.
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克隆多克隆
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同种型IgG
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研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab85452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 155 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
靶标
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功能Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
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组织特异性Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
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序列相似性Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
结构域Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
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翻译后修饰Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function. -
细胞定位Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 8202 Human
- Entrez Gene: 84584 Rat
- Omim: 601937 Human
- SwissProt: Q9Y6Q9 Human
- SwissProt: Q9EPU2 Rat
- Unigene: 592142 Human
- Unigene: 20691 Rat
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别名
- ACTR antibody
- AIB 1 antibody
- AIB-1 antibody
see all
Anti-SRC3 antibody 图像
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Western blot - Anti-SRC3 antibody (ab85452)
Predicted band size : 155 kDa
Western blot analysis of MCF7 cell lysate labeling SRC3 with ab85452 at 1.0µg/ml. -
Western blot - KAT13B / AIB1 antibody (ab85452)Anti-SRC3 antibody (ab85452) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 155 kDa
Observed band size : 155 kDa
Gel concentration: P6-18% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SRC3 antibody(ab85452)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SRC3 with ab85452 at 5.0µg/ml.
Anti-SRC3 antibody (ab85452)参考文献
ab85452 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"