概述

  • 产品名称Anti-SRC3抗体[0.T.198]
    参阅全部 SRC3 一抗
  • 描述
    小鼠单克隆抗体[0.T.198] to SRC3
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 605-1294 of Human NCOA3.

  • 阳性对照
    • RIPA lysates from BG-1 ovarian cancer cells.
  • 常规说明Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

应用

Our Abpromise guarantee covers the use of ab14139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 170 kDa).
IP Use 10µl for 10500 cells.
IHC-P Use at an assay dependent dilution.

靶标

  • 功能Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
  • 组织特异性Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • 序列相似性Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
  • 翻译后修饰Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.
  • 细胞定位Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACTR antibody
    • AIB 1 antibody
    • AIB-1 antibody
    • AIB1 antibody
    • Amplified in breast cancer 1 antibody
    • Amplified in breast cancer 1 protein antibody
    • bHLHe42 antibody
    • CAGH 16 antibody
    • CAGH16 antibody
    • CBP interacting protein antibody
    • CBP-interacting protein antibody
    • Class E basic helix-loop-helix protein 42 antibody
    • CTG 26 antibody
    • CTG26 antibody
    • KAT13B / AIB1 antibody
    • MGC141848 antibody
    • NCOA 3 antibody
    • NCoA-3 antibody
    • Ncoa3 antibody
    • NCOA3_HUMAN antibody
    • Nuclear receptor coactivator 3 antibody
    • pCIP antibody
    • RAC 3 antibody
    • RAC-3 antibody
    • RAC3 antibody
    • Receptor associated coactivator 3 antibody
    • Receptor-associated coactivator 3 antibody
    • SRC 3 antibody
    • SRC-3 antibody
    • SRC3 antibody
    • Steroid receptor coactivator protein 3 antibody
    • Thyroid hormone receptor activator molecule 1 antibody
    • TNRC14 antibody
    • TNRC16 antibody
    • TRAM 1 antibody
    • TRAM-1 antibody
    • TRAM1 antibody
    see all

Anti-SRC3 antibody [0.T.198] 图像

  • Superficial spreading type level IV malignant melanoma from the skin of the back, with microsatellite formation, possible lymphatic vascular invasion, low mitotic rate and moderate tumor infiltrating lymphocytes. Ab14139 used at a dilution of 1:25.

Anti-SRC3 antibody [0.T.198] (ab14139)参考文献

This product has been referenced in:
  • Gould Rothberg BE  et al. Tissue biomarkers for prognosis in cutaneous melanoma: a systematic review and meta-analysis. J Natl Cancer Inst 101:452-74 (2009). Read more (PubMed: 19318635) »
  • Rangel J  et al. Prognostic significance of nuclear receptor coactivator-3 overexpression in primary cutaneous melanoma. J Clin Oncol 24:4565-9 (2006). IHC-P ; Human . Read more (PubMed: 17008696) »

See all 2 Publications for this product

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Thank you for your enquiry. I am sorry I couldn't get any information about the specific antigen retrival method used for the image seen on the datasheet. I would assume a general antigen retrival method was used. Please refer to our website's IHC-P...

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