Anti-Src (phospho Y418)抗体(ab4816)

概述

  • 产品名称Anti-Src (phospho Y418)抗体
    参阅全部 Src 一抗
  • 描述
    兔多克隆抗体to Src (phospho Y418)
  • 经测试应用适用于: IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
  • 免疫原

    Synthetic peptide derived from the region of Src that contains tyrosine 418 (tyrosine 419 including the initiating methionine). The sequence is conserved in human, mouse, rat, chicken and frog.

  • 阳性对照
    • Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein.
  • 常规说明


    Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Src, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Src-derived peptide that is phosphorylated at tyrosine 418.
  • Primary antibody说明Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 21901128
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 细胞定位Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 形式This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 别名
    • ASV antibody
    • Avian sarcoma virus antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC 2.7.10.2 antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

Anti-Src (phospho Y418) antibody 图像

  • Immunohistochemical analysis of preimplantation stage embryos collected from the reproductive tracts of superovulated female mice. (A) Blastocysts exposed to secondary antibody only, (B) blastocysts cultured in KSOMaa medium, (C) blastocysts cultured in 10-3 M Ouabain, (D) blastocysts cultured in 10-4 M Ouabain.

    Src (phospho Y418) was stained using ab4816 (green) in panels B-D, at 1/100 dilution. A FITC-conjugated IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).


  • Predicted band size : 60 kDa

    Dr. Michael Schaller, Dept. of Cell Bio. & Anat., Univ. of North Carolina, Chapel Hill, NC.

    Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].

     

     

     

    Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].

Anti-Src (phospho Y418) antibody (ab4816)参考文献

This product has been referenced in:
  • Nikonova AS  et al. Nedd9 restrains renal cystogenesis in Pkd1-/- mice. Proc Natl Acad Sci U S A 111:12859-64 (2014). Read more (PubMed: 25139996) »
  • Haidari M  et al. Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-ß-dependent vascular endothelial cadherin tyrosine phosphorylation. Cardiovasc Diabetol 13:112 (2014). Human . Read more (PubMed: 25027642) »

See all 12 Publications for this product

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I am very pleased to hear you would like to accept our offer and test ab4816 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the ex...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela kyoto beta)
Loading amount 15 µg
Specification Hela kyoto beta
Treatment siRNA knock down for 48 h
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 4°C
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Verified customer

提交于 Aug 05 2010

I just received some good news. I was able to obtain the specific concentration for 2 of our current lots, which are: lot 442382, 0.4 mg/mL lot 502322, 0.5 mg/mL

Thank you for your enquiry. I have performed a sequence alignment of the immunogen sequence and I can tell you that the corresponding C.elegans amino acid sequence has 8 out of 14 amino acid residues in common. However it is most dissimilar in the r...

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Thank you very much for your patience. The batches of ab4816 and ab4817 that we currently have in stock are at a concentration of 0.25 mg/mL. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"