Anti-Src (phospho Y418)抗体(ab47411)


  • 产品名称Anti-Src (phospho Y418)抗体
    参阅全部 Src 一抗
  • 描述
    兔多克隆抗体to Src (phospho Y418)
  • 特异性This antibody detects endogenous levels of Src only when phosphorylated at tyrosine 418.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Src around the phosphorylation site of tyrosine 418 (N-E-YP-T-A).

  • 阳性对照
    • Human colon carcinoma tissue; COLO205 cell extracts



Our Abpromise guarantee covers the use of ab47411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.


  • 功能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 细胞定位Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 形式This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 别名
    • ASV antibody
    • Avian sarcoma virus antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

Anti-Src (phospho Y418) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue in the presence (right) and absence (left) of blocking phosphopeptide, using ab47411 at 1/50 dilution.
  • All lanes : Anti-Src (phospho Y418) antibody (ab47411) at 1/500 dilution

    Lane 1 : COLO205 cell extracts with serum
    Lane 2 : COLO205 cell extracts

    Predicted band size : 60 kDa

Anti-Src (phospho Y418) antibody (ab47411)参考文献

This product has been referenced in:
  • Donahue TR  et al. Integrative survival-based molecular profiling of human pancreatic cancer. Clin Cancer Res 18:1352-63 (2012). IHC-P ; Human . Read more (PubMed: 22261810) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat pH6
Sample Mouse Tissue sections (kidney)
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde

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Verified customer

提交于 Aug 20 2014

Application Western blot
Sample Rat Tissue lysate - whole (Kidney)
Loading amount 10 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing (10% Gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

提交于 Feb 12 2013

ab38497 : DISCOUNT CODE: 41002

ab47411: DISCOUNT CODE: 41864

Expiration date:09. October 2012

I am very pleased to hear your customer would like to accept our offer and test ab38497 and ab47411in mouse.

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We do not stock small sample sizes for our products. Only sizes noted on the online product datasheets are available. However, it seems you are interested in using ab47411 in IHC on paraffin embedded sections of hu...

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For ab4817, ab32078 the immuinogen peptide are unfortunately not available.

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