Anti-Src (phospho S75)抗体(ab79308)


  • 产品名称Anti-Src (phospho S75)抗体
    参阅全部 Src 一抗
  • 描述
    兔多克隆抗体to Src (phospho S75)
  • 特异性Detects Src only when phosphorylated at serine 75
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human Src around the phosphorylation site of serine 75 (VTSPPQ)

  • 阳性对照
    • IHC-P: Human brain tissue WB: extracts from COLO cells treated with EGF



Our Abpromise guarantee covers the use of ab79308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 60 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.


  • 功能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 细胞定位Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 形式This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 别名
    • ASV antibody
    • Avian sarcoma virus antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

Anti-Src (phospho S75) antibody 图像

  • All lanes : Anti-Src (phospho S75) antibody (ab79308) at 1/1000 dilution

    Lane 1 : extracts from COLO cells (treated with EGF, 200ng/ml, 30mins)
    Lane 2 : extracts from COLO cells (treated with EGF, 200ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Left panel: immunohistochemistry analysis of paraffin-embedded Human brain tissue with ab79308 at 1/100 dilution. Right panel: same, after pre-incubation with the immunizing peptide
  • ab79308 staining Src (phospho S75) in Dog osteosarcoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with acetone and blocked with 2% serum for 1 hour; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50 in serum) for 14 hours at 4°C. A Biotinylated Goat anti-rabbit IgG (1/400) was used as the secondary antibody.

    See Abreview

Anti-Src (phospho S75) antibody (ab79308)参考文献

ab79308 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (osteosarcoma)
Specification osteosarcoma
Fixative Acetone
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%

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提交于 Jun 18 2012

I am very pleased to hear you would like to accept our offer these antibodiesindog. This code will give you: 1 freeprimary antibodybefore the expiration date for each antibody tested. To redeem this offer, please submit an Abreview for dog for eac...

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Thank you for your reply and the additional information which is very helpful. The fact that the total protein gives you the same band pattern might indicate that these bands could be real for your rat samples. Which antibody did you use to detect t...

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Thank you for contacting us and for including the WB image. The band shown on our datasheet runs at about 55 to 60 kDa and was blocked with the immunizing peptide. Only human samples have so far been tested with this antibody. This antibody detec...

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