概述

  • 产品名称Anti-SRA1抗体
    参阅全部 SRA1 一抗
  • 描述
    兔多克隆抗体to SRA1
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 50 and 100 of human SRA1 (NP_001030312.1)

  • 阳性对照
    • HeLa, MCF7 and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab72552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
  • 组织特异性Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
  • 序列相似性Belongs to the SRA1 family.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC87674 antibody
    • PP7684 antibody
    • SR A1 antibody
    • SRA antibody
    • SRA1 antibody
    • SRA1_HUMAN antibody
    • SRAP antibody
    • Steroid receptor coactivator antibody
    • Steroid receptor RNA activator 1 (complexes with NCOA1) antibody
    • Steroid receptor RNA activator 1 antibody
    • Steroid receptor RNA activator protein antibody
    • STRAA1 antibody
    see all

Anti-SRA1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SRA1 with ab72552 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-SRA1 antibody (ab72552) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 20 µg
    Lane 2 : HeLa whole cell lysate at 4 µg
    Lane 3 : MCF7 whole cell lysate at 20 µg
    Lane 4 : 293T whole cell lysate at 20 µg


    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Detection of Human SRA1 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72552 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

Anti-SRA1 antibody (ab72552)参考文献

This product has been referenced in:
  • McKay DB  et al. Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. J Mol Biol 426:1766-85 (2014). IF . Read more (PubMed: 24486609) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab72552.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"