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In western blot the antibody recognizes 4 major bands of 130, 62, 45 and 22 kDa in human brain homogenate. The antibody may also recognize some proteins containing SQSTM1 domains from other species as 80, 62 and 47 kDa proteins are identified in rat sympathetic ganglia. Preincubation of the antibody with the immunizing peptide abolishes all reactivity in both human brain homogenate and rat sympathitic ganglia.
Our Abpromise guarantee covers the use of ab31545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||1/500 - 1/2000.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 47, 50 kDa (predicted molecular weight: 47 kDa).|
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