Anti-SQSTM1 / p62抗体(ab31545)

概述

  • 产品名称Anti-SQSTM1 / p62抗体
    参阅全部 SQSTM1 / p62 一抗
  • 描述
    羊多克隆抗体to SQSTM1 / p62
  • 特异性ab31545 recognises SQSTM1/p62 in normal and diseased human brain.
  • 经测试应用适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from part of human Ubiquitin Interactive Motif (UIM) derived from p62 protein, also known as SQSTM1. Amino acid sequence: PPEADPRLIE SLSQMLSMGF SDEGGWLTRL LQTKNYDIGA

  • 阳性对照
    • Human brain homogenate
  • 常规说明


    In western blot the antibody recognizes 4 major bands of 130, 62, 45 and 22 kDa in human brain homogenate. The antibody may also recognize some proteins containing SQSTM1 domains from other species as 80, 62 and 47 kDa proteins are identified in rat sympathetic ganglia. Preincubation of the antibody with the immunizing peptide abolishes all reactivity in both human brain homogenate and rat sympathitic ganglia.

性能

应用

Our Abpromise guarantee covers the use of ab31545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
IHC-Fr 1/500 - 1/2000.
WB Use at an assay dependent dilution. Detects a band of approximately 47, 50 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.
  • 序列相似性Contains 1 OPR domain.
    Contains 1 UBA domain.
    Contains 1 ZZ-type zinc finger.
  • 结构域The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
    The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
    The ZZ-type zinc finger mediates the interaction with RIPK1.
  • 翻译后修饰Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.
  • 细胞定位Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A170 antibody
    • EBI 3 associated protein of 60 kDa antibody
    • EBI 3 associated protein p60 antibody
    • EBI3 associated protein of 60 kDa antibody
    • EBI3 associated protein p60 antibody
    • EBI3-associated protein of 60 kDa antibody
    • EBIAP antibody
    • FTDALS3 antibody
    • MGC127197 antibody
    • ORCA antibody
    • OSF-6 antibody
    • Osi antibody
    • OSIL antibody
    • Oxidative stress induced like antibody
    • p60 antibody
    • p62 antibody
    • p62B antibody
    • Paget disease of bone 3 antibody
    • PDB 3 antibody
    • PDB3 antibody
    • Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
    • Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
    • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
    • PKC-zeta-interacting protein antibody
    • Protein kinase C-zeta-interacting protein antibody
    • Sequestosome 1 antibody
    • Sequestosome-1 antibody
    • SQSTM 1 antibody
    • SQSTM_HUMAN antibody
    • Sqstm1 antibody
    • STAP antibody
    • STONE14 antibody
    • Ubiquitin binding protein p62 antibody
    • Ubiquitin-binding protein p62 antibody
    • ZIP 3 antibody
    • ZIP antibody
    • ZIP3 antibody
    see all

Anti-SQSTM1 / p62 antibody 图像

  • All lanes : Anti-SQSTM1 / p62 antibody (ab31545) at 1/500 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 + 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • ab31545 staining SQSTM1 / p62 in murine spinal cord sections by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 4% paraformaldehyde, blocked with 10% goat serum for 2 hours at 25°C and then incubated with ab31545 at a 1/500 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated donkey anti-sheep (H+L) polyclonal used at a 1/1000 dilution.

    See Abreview

Anti-SQSTM1 / p62 antibody (ab31545)参考文献

This product has been referenced in:
  • Campbell GR  et al. Human Immunodeficiency Virus Type 1 Nef Inhibits Autophagy through Transcription Factor EB Sequestration. PLoS Pathog 11:e1005018 (2015). Read more (PubMed: 26115100) »
  • Puustinen P  et al. CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation. J Cell Biol 204:713-27 (2014). Human . Read more (PubMed: 24590173) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Teresa Muñoz-Galdeano

Verified customer

提交于 Feb 04 2012

Thank you for contacting us. Unfortunately, we don’t have specific data regarding the reactivity of this antibody in Drosophila. It has only been tested in mammalian species. Could you please specify the source where you found the reference to the reac...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Kc cells)
Loading amount 1e+006 cells
Specification Kc cells
Treatment RNAi against indicated gene for three days
Gel Running Conditions Reduced Denaturing (8% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 23 2010

Thank you for your enquiry. The synthetic peptide used to raise ab31545 was derived from part of human Ubiquitin Interactive Motif (UIM) derived from p62 protein, also known as SQSTM1. The amino acid sequence is as follows: PPEADPRLIE SLSQMLSMGF SD...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"