概述

  • 产品名称
    Anti-SPZ1抗体
  • 描述
    兔多克隆抗体to SPZ1
  • 宿主
    Rabbit
  • 特异性
    This antibody reacts with SPZ1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    A region within synthetic peptide: FKHTTRATEG TIRKPKILRS PDVCFTKKAR NNRFNARVAK KSLVGKRRTV, corresponding to C terminal amino acids 325-374 of Mouse SPZ1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • NIH3T3 lysate

性能

应用

Our Abpromise guarantee covers the use of ab50870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay: 1/312500.

靶标

  • 相关性
    SPZ1 is a novel bHLH-Zip protein.
  • 细胞定位
    Cytoplasmic and Nuclear
  • 数据库链接
    • 别名
      • BHLH Zip transcription factor SPZ1 antibody
      • FLJ25709 antibody
      • MGC133987 antibody
      • NYD TSP1 antibody
      • PP1C gamma 2 antibody
      • PP1C gamma antibody
      • PPP1R148 antibody
      • Protein phosphatase 1, regulatory subunit 148 antibody
      • Spermatogenic leucine zipper 1 antibody
      • Spermatogenic leucine zipper protein 1 antibody
      • Spermatogenic Zip 1 antibody
      • Spz1 antibody
      • Testis secretory sperm-binding protein Li 232m antibody
      • Testis specific protein 1 antibody
      • Testis specific protein NYD TSP1 antibody
      • TSP1 antibody
      see all

    图片

    • Western blot - Anti-SPZ1 antibody (ab50870)
      Western blot - Anti-SPZ1 antibody (ab50870)
      Anti-SPZ1 antibody (ab50870) at 2.5 µg/ml + NIH3T3 lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 49 kDa
      Observed band size: 42 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    文献

    ab50870 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab50870？请让我们知道，以便我们可以引用本数据表中的参考文章。

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