Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Titre using peptide based assay: 1/312500.
Anti-SPZ1 antibody 图像
Western blot - SPZ1 antibody (ab50870)
Anti-SPZ1 antibody (ab50870) at 2.5 µg/ml + NIH3T3 lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
49 kDaObserved band size :
42 kDa (why is the actual band size different from the predicted?
)Additional bands at :
28 kDa. We are unsure as to the identity of these extra bands.
Anti-SPZ1 antibody (ab50870)参考文献
has not yet been referenced specifically in any publications.
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