Anti-SPTLC1抗体(ab84585)

概述

  • 产品名称Anti-SPTLC1抗体
    参阅全部 SPTLC1 一抗
  • 描述
    兔多克隆抗体to SPTLC1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (DLERLLKEQE IEDQKNPRKA RVTRRFIVVE GLYMNTGTIC PLPELVKLKY) of Human SPTLC1 (NP_006406).

  • 阳性对照
    • Human fetal brain lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: JEG3.

应用

Our Abpromise guarantee covers the use of ab84585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Serine palmitoyltransferase (SPT). The heterodimer formed with LCB2 (SPTLC2 or SPTLC3) constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SSSPTB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SSSPTB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference.
  • 组织特异性Widely expressed. Not detected in small intestine.
  • 通路Lipid metabolism; sphingolipid metabolism.
  • 疾病相关Defects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A) [MIM:162400]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations.
  • 序列相似性Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSAN antibody
    • HSAN1 antibody
    • HSN1 antibody
    • LBC1 antibody
    • LCB 1 antibody
    • LCB1 antibody
    • Long chain base biosynthesis protein 1 antibody
    • MGC14645 antibody
    • Serine C palmitoyltransferase antibody
    • Serine palmitoyl CoA transferase 1 antibody
    • Serine palmitoyltransferase 1 antibody
    • Serine palmitoyltransferase long chain base subunit 1 antibody
    • Serine palmitoyltransferase subunit 1 antibody
    • Serine-palmitoyl-CoA transferase 1 antibody
    • SPT 1 antibody
    • SPT1 antibody
    • SPTC1_HUMAN antibody
    • SPTI antibody
    • SPTLC 1 antibody
    • SPTLC1 antibody
    see all

Anti-SPTLC1 antibody 图像

  • ICC/IF image of ab84585 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84585 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-SPTLC1 antibody (ab84585) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-SPTLC1 antibody (ab84585)参考文献

ab84585 has not yet been referenced specifically in any publications.

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