Anti-SPTLC1抗体(ab232847)
Key features and details
- Rabbit polyclonal to SPTLC1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-SPTLC1抗体
参阅全部 SPTLC1 一抗 -
描述
兔多克隆抗体to SPTLC1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Recombinant fragment -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Mouse SPTLC1 aa 100 to the C-terminus. Two N-terminal tags (Expressed in E.coli).
Database link: O35704 -
阳性对照
- IHC-P: Mouse kidney, uterus and liver tissue. WB: Mouse liver, lung and brain tissue; Recombinant mouse SPTLC1 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232847于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
靶标
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功能
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB2 (SPTLC2 or SPTLC3) constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SSSPTB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SSSPTB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. -
组织特异性
Widely expressed. Not detected in small intestine. -
通路
Lipid metabolism; sphingolipid metabolism. -
疾病相关
Defects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A) [MIM:162400]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations. -
序列相似性
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 268656 Mouse
- SwissProt: O35704 Mouse
- Unigene: 240336 Mouse
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别名
- HSAN antibody
- HSAN1 antibody
- HSN1 antibody
see all
图片
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Formalin-fixed, paraffin-embedded mouse uterus tissue stained for SPTLC1 using ab232847 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded mouse kidney tissue stained for SPTLC1 using ab232847 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded mouse liver tissue stained for SPTLC1 using ab232847 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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All lanes : Anti-SPTLC1 antibody (ab232847) at 1 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution -
Anti-SPTLC1 antibody (ab232847) at 1 µg/ml + Recombinant mouse SPTLC1 protein
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab232847 被引用在 1 文献中.
- Zhu WK et al. Decreased SPTLC1 expression predicts worse outcomes in ccRCC patients. J Cell Biochem 121:1552-1562 (2020). PubMed: 31512789