重组Anti-SPTBN1抗体[EPR5869] (ab124888)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5869] to SPTBN1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SPTBN1抗体[EPR5869]
参阅全部 SPTBN1 一抗 -
描述
兔单克隆抗体[EPR5869] to SPTBN1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human SPTBN1 aa 1900-2000. The exact sequence is proprietary.
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阳性对照
- Human kidney tissue; SH-SY5Y, HeLa, Caco 2 and Jurkat whole cell lysate (ab7899).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 4.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5869 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124888于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 275 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 275 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
靶标
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功能
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
组织特异性
Isoform 2 is present in brain, lung and kidney (at protein level). -
序列相似性
Belongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 1 PH domain.
Contains 17 spectrin repeats. -
翻译后修饰
Isoform 2 is phosphorylated on Ser-8 and Ser-10. -
细胞定位
Cell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 6711 Human
- Entrez Gene: 20742 Mouse
- Entrez Gene: 305614 Rat
- Omim: 182790 Human
- SwissProt: Q01082 Human
- SwissProt: Q62261 Mouse
- Unigene: 503178 Human
- Unigene: 705692 Human
see all -
别名
- Beta G spectrin antibody
- Beta II spectrin antibody
- Beta spectrin 2 antibody
see all
图片
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ab124888, at 1/250 dilution, staining SPTBN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 275 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124888 被引用在 2 文献中.
- Zhao F et al. Scleral HIF-1a is a prominent regulatory candidate for genetic and environmental interactions in human myopia pathogenesis. EBioMedicine 57:102878 (2020). PubMed: 32652319
- Syrbe S et al. Delineating SPTAN1 associated phenotypes: from isolated epilepsy to encephalopathy with progressive brain atrophy. Brain 140:2322-2336 (2017). PubMed: 29050398