Suppressor of Ty (S.cerevisiae) 6 homolog antibody
Suppressor of Ty 6 homolog (S. cerevisiae) antibody
Tat cotransactivator 2 protein antibody
Tat CT2 protein antibody
Tat-cotransactivator 2 protein antibody
Tat-CT2 protein antibody
Transcription elongation factor SPT6 antibody
Western blot - Spt6 antibody (ab32820)
Lane 1 : Marker Lanes 2-4 : Anti-Spt6 antibody (ab32820) at 1/500 dilution Lane 5 : High Mark marker
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg Lane 3 :Jurkat whole cell lysate (ab7899) at 20 µg Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Secondary Lanes 2-4 : IR Dye 680 Conjugated Goat anti Rabbit IgG (H+L) at 1/21250 dilution
ab32820 staining Spt6 in human gall bladder, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab32820 (1/30 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab32820 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Spt6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Spt6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32820. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 230kDa: Spt6
Soboleva TA et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet13:e1006633 (2017).
Read more (PubMed: 28234895) »
Dembowski JA et al. Replication-Coupled Recruitment of Viral and Cellular Factors to Herpes Simplex Virus Type 1 Replication Forks for the Maintenance and Expression of Viral Genomes. PLoS Pathog13:e1006166 (2017).
Read more (PubMed: 28095497) »