概述

  • 产品名称Anti-SPT3抗体
    参阅全部 SPT3 一抗
  • 描述
    兔多克隆抗体to SPT3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: GARVITPEDL LFLMRKDKKK LRRLLKYMFI RDYKSKIVKG IDEDDLLEDK, corresponding to amino acids 74-123 of Human SPT3

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab66892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 23, 60 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

Anti-SPT3 antibody 图像

  • Anti-SPT3 antibody (ab66892) at 0.4 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry of Human Liver cell lysate tissue at an antibody concentration of 4-8µg/ml using anti-SUPT3H antibody (ab66892)

Anti-SPT3 antibody (ab66892)参考文献

ab66892 has not yet been referenced specifically in any publications.

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