- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-SPRR1b抗体
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描述兔多克隆抗体to SPRR1b
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宿主Rabbit
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经测试应用适用于: IHC-Fr, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
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免疫原
Synthetic peptide corresponding to amino acids 48-89 (PCHPKVPEPCHPKVPEPCQPKVPEPCHPKVPEPCPSIVTPAPAQQKTKQ K) of human SPRR1b (NP_003116).
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阳性对照
- Jurkat cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液Constituents: 97% PBS, 2% Sucrose
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Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab123237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | 1/50. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
靶标
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功能Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage.
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组织特异性Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
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序列相似性Belongs to the cornifin (SPRR) family.
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发展阶段Expressed during differentiation of squamous cells.
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翻译后修饰The N-terminus is blocked.
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细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 6699 Human
- Entrez Gene: 20754 Mouse
- Entrez Gene: 685912 Rat
- Omim: 182266 Human
- SwissProt: P22528 Human
- SwissProt: Q62267 Mouse
- Unigene: 1076 Human
- Unigene: 140151 Mouse
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别名
- 14.9 kDa pancornulin antibody
- Cornifin antibody
- Cornifin B antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-SPRR1b antibody (ab123237)Immunohistochemistry (Frozen sections) analysis of mouse eyeball sections labelling SPRR1b with ab123237 at 1:50. An Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:50) was used as the secondary antibody.
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Western blot - Anti-SPRR1b antibody (ab123237)Anti-SPRR1b antibody (ab123237) at 1 µg/ml + Jurkat cell lysate at 10 µg
Secondary
Goat anti-Rabbit IgG HRP
Predicted band size: 10 kDa
Gel concentration: 10-20%.
文献
This product has been referenced in:
- Fang Z et al. Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins. BMC Cancer 16:706 (2016). WB ; Human . Read more (PubMed: 27586393) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"