Anti-SPRR1a抗体(ab125374)

概述

  • 产品名称
    Anti-SPRR1a抗体
  • 描述
    兔多克隆抗体to SPRR1a
  • 经测试应用
    适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
  • 免疫原

    Synthetic peptide corresponding to Human SPRR1a aa 40-89 (C terminal).
    Sequence:

    PCHPKVPEPCHPKVPEPCQPKVPEPCQPKVPEPCPSTVTPAPAQQKTKQK


    Database link: P35321

  • 阳性对照
    • HT1080 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab125374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    The ability of neurons to regenerate an axon after injury is determined by both the surrounding environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A (SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction of SPRR1A function restricts axonal outgrowth.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • 19 kDa pancornulin antibody
    • Cornifin A antibody
    • Cornifin alpha antibody
    • CornifinA antibody
    • Small proline rich protein 1A antibody
    • Small proline rich protein IA antibody
    • spr antibody
    • SPR IA antibody
    • SPRK antibody
    • SPRR 1a antibody
    • SPRR1A protein antibody
    see all

图片

  • Anti-SPRR1a antibody (ab125374) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Predicted band size : 10 kDa
  • ab125374 staining SPRR1a in Human skin tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with acetone and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/300 in 1% BSA + 10% goat serum) for 16 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

文献

ab125374 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Yuan Cao

Verified customer

提交于 Jul 27 2012

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I am very pleased to hear you would like to accept our offer and test ab125374 in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the e...

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