Anti-Sprouty 4 (phospho Y75)抗体(ab103114)

概述

  • 产品名称Anti-Sprouty 4 (phospho Y75)抗体
    参阅全部 Sprouty 4 一抗
  • 描述
    兔多克隆抗体to Sprouty 4 (phospho Y75)
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 57-86 from Tyrosine 75 region of Human Sprouty 4

  • 阳性对照
    • K-562 cell lysate. Human brain tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab103114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 32 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
  • 序列相似性Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • 结构域The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • 细胞定位Cytoplasm. Cell projection > ruffle membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein sprouty homolog 4 antibody
    • Sprouty homolog 4 antibody
    • sprouty homolog 4 (Drosophila) antibody
    • SPRY 4 antibody
    • Spry-4 antibody
    • Spry4 antibody
    • SPY4_HUMAN antibody
    see all

Anti-Sprouty 4 (phospho Y75) antibody 图像

  • ab103114, at 1/50 dilution, staining Sprouty 4 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
  • Anti-Sprouty 4 (phospho Y75) antibody (ab103114) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 32 kDa

Anti-Sprouty 4 (phospho Y75) antibody (ab103114)参考文献

ab103114 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jul 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"