This fast track antibody is not yet fully characterized. It is subject to these terms and conditions



  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


  • 应用说明Tested in peptide ELISA.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody has been tested by Western blot against cell lysates. We believe that Sprouty 4 is represented by the doublet at ~ 35kDa. We believe that this protein may require IP before Western blotting, corresponding to results seen in Sasaki et al. We have not yet succeeded in peptide blocking the bands detected with this antibody.

  • 靶标

    Anti-Sprouty 4 antibody 图像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Western blot using ab7513 at 1/500.
      Lane 1: Hela nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
      Exposure time: 2minutes.
      Lysates at 5µg per lane.

      We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.

    • All lanes : Anti-Sprouty 4 antibody (ab7513) at 1/100 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : SW13 whole cell lysate
      Lane 3 : C2C12 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.
    • ab7513 at 2.5 µg/ml staining human heart tissue.

    • ab7513 staining tumour cells in human liver tissue.  Brown color indicates presence of protein, blue color shows cell nuclei.

    Anti-Sprouty 4 antibody (ab7513)参考文献

    This product has been referenced in:
    • Barbáchano A  et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene 29:4800-13 (2010). WB ; Human . Read more (PubMed: 20543868) »
    • Price KL  et al. Microarray interrogation of human metanephric mesenchymal cells highlights potentially important molecules in vivo. Physiol Genomics 28:193-202 (2007). IHC-P ; Human . Read more (PubMed: 16985006) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Melanoma)
    Loading amount 500 µg
    Specification Melanoma
    Gel Running Conditions Reduced Denaturing (15 %)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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    提交于 Sep 06 2010

    Thank you for your enquiry. Ab7513 is a Fast-Track antibody and has not yet been fully characterized. Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testi...

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