Anti-Sprouty 2抗体- Aminoterminal end (ab50317)

概述

  • 产品名称Anti-Sprouty 2抗体- Aminoterminal end
    参阅全部 Sprouty 2 一抗
  • 描述
    兔多克隆抗体to Sprouty 2 - Aminoterminal end
  • 经测试应用适用于: IHC-P, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Dog, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    HERLHGLPEHRQPPRLQPSQ

    corresponding to amino acids 79-98 of Mouse Sprouty 2, conjugated to KLH by a C terminal Cysteine residue linker.

  • 阳性对照
    • WB: Extracts of MDCK cells. IP: extracts of 293-T cells transfected with human Sprouty 2. ICC/IF: 293-T cells transfected with human Sprouty 2.

性能

应用

Our Abpromise guarantee covers the use of ab50317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 19581591
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 35 kDa.
IP Use a concentration of 5 - 10 µg/ml.
ICC/IF Use a concentration of 2 - 4 µg/ml.

靶标

  • 功能May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
  • 序列相似性Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • 结构域The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • 细胞定位Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSPRY 2 antibody
    • hSPRY2 antibody
    • MGC23039 antibody
    • Protein sprouty homolog 2 antibody
    • Sprouty 2 antibody
    • Sprouty homolog 2 (Drosophila) antibody
    • Sprouty homolog 2 antibody
    • Sprouty2 antibody
    • Spry 2 antibody
    • Spry-2 antibody
    • SPRY2 antibody
    • SPY2_HUMAN antibody
    see all

Anti-Sprouty 2 antibody - Aminoterminal end 图像

  • ab50317 staining Sprouty 2 in Mouse 8-cell stage pre-implantation embryo by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in 1x PBS and blocked with 3% BSA for 4 hours 30 minutes at 4°C. Samples were incubated with primary antibody (1/300 in 1 x PBS + 3% BSA + 0.15% Tween-20) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    The image of an 8-cell stage pre-implantation Mouse embryo that was injected with wtSpry2 (N-terminally tagged with YFP) mRNA at 2-cell stage (in green) and stained with anti-Spry2 antibody (ab50317) (in red). Nuclei are stained with DAPI (in blue).

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  • All lanes : Anti-Sprouty 2 antibody - Aminoterminal end (ab50317) at 1 µg/ml

    Lane 1 : 293-T cells transfected with Sprouty-2
    Lane 2 : C2C12 myoblasts (human)
    Lane 3 : Jurkat (human)
    Lane 4 : NIH3T3 (mouse)
    Lane 5 : Sw48 (human)
    Lane 6 : MDCK (canine)

    Secondary
    Goat Anti-Rabbit IgG-Peroxidase conjugate

    Predicted band size : 35 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa (possible dimer).
  • ab50317 staining Sprouty 2 in Mouse bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 hours at 20°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/500) for 24 hours at 4°C. An undiluted HRP-conjugated Mouse anti-rabbit polyclonal was used as the secondary antibody.

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Anti-Sprouty 2 antibody - Aminoterminal end (ab50317)参考文献

This product has been referenced in:
  • Ma X  et al. Interaction of the oncogenic miR-21 microRNA and the p53 tumor suppressor pathway. Carcinogenesis 34:1216-23 (2013). Mouse . Read more (PubMed: 23385064) »
  • Andreadi C  et al. The intermediate-activity (L597V)BRAF mutant acts as an epistatic modifier of oncogenic RAS by enhancing signaling through the RAF/MEK/ERK pathway. Genes Dev 26:1945-58 (2012). Read more (PubMed: 22892241) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 4 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Mouse Cell (8-cell stage mouse pre-implantation embryo)
Specification 8-cell stage mouse pre-implantation embryo
Permeabilization Yes - 0.5% Triton X-100 in 1xPBS
Fixative Paraformaldehyde
Username

Aleksandar Mihajlovic

Verified customer

提交于 Sep 16 2013


On the datasheet for ab50317, we recommend storage conditions of 4oC only for short term (1-2 weeks) with longer-term storage at -20 or -80oC. Since this antibody has been stored at 4oC for over 6 months, I will be unable to refund or replace the ...

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I am sorry to hear that you have been experiencing issues when using this antibody. I see that the lot you referenced of this antibody was purchased on 6/9/12 and have a few questions since it has been awhile since your purchase.
1. How has th...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I have issued a free of charge replacement.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mammary epithelial cells)
Loading amount 50 µg
Specification mammary epithelial cells
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Bladder)
Specification Bladder
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Imran Ahmad

Verified customer

提交于 Nov 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (skin wound)
Loading amount 15 µg
Specification skin wound
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Precedia Massey

Verified customer

提交于 Feb 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"