概述

  • 产品名称
    Anti-Sprouty 1抗体
    参阅全部 Sprouty 1 一抗
  • 描述
    兔多克隆抗体to Sprouty 1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region of Human Sprouty 1.

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明
    The reactivity to rat seems to be batch dependent.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

靶标

图片

  • Anti-Sprouty 1 antibody (ab75492) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Sprouty 1 antibody (ab75492) at 5 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish liver homogenate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

实验方案

文献

This product has been referenced in:

See all 3 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293 cells transfected with plasmid expressing)
Loading amount
20 µg
Specification
HEK293 cells transfected with plasmid expressing
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 01 2012

Thank you very much for your answer. After looking through the information provided, I would like to point out the following: 1.) We do not have tested rat as a species, and obviously therefore rat is not a batch tested species neither....

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Thank you for contacting us. I am very sorry to hear you are experiencing difficulties with the new batches of this product. We take product complaints very seriously, and investigate every product and batch that we feel may not be performing correctly...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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