Anti-Sprouty 1抗体(ab56670)


  • 产品名称Anti-Sprouty 1抗体
    参阅全部 Sprouty 1 一抗
  • 描述
    小鼠单克隆抗体to Sprouty 1
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-111 of Human Sprouty 1

  • 阳性对照
    • HepG2 cell lysate, heart tissue


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab56670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 功能May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
  • 序列相似性Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • 结构域The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • 细胞定位Cytoplasm. Membrane. Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSPRY1 antibody
    • Protein sprouty homolog 1 antibody
    • Sprouty homolog 1 antagonist of FGF signaling antibody
    • Sprouty homolog 1 antibody
    • Spry-1 antibody
    • Spry1 antibody
    • SPY1_HUMAN antibody
    see all

Anti-Sprouty 1 antibody 图像

  • Overlay histogram showing HepG2 cells stained with ab56670 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56670, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Sprouty 1 antibody (ab56670) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.

  • Predicted band size : 35 kDa
    Sprouty 1 antibody (ab56670) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

Anti-Sprouty 1 antibody (ab56670)参考文献

ab56670 has not yet been referenced specifically in any publications.

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