概述

  • 产品名称Anti-SPRED1抗体
    参阅全部 SPRED1 一抗
  • 描述
    兔多克隆抗体to SPRED1
  • 特异性ab77079 is predicted to have no cross-reactivity to SPRED2 or SPRED3.
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic 14 amino acid peptide from near the center of human SPRED1 (NP_689807).

  • 阳性对照
    • Human brain tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549.

性能

应用

Our Abpromise guarantee covers the use of ab77079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow.
  • 组织特异性Weakly expressed in embryonic cell line (HEK-293).
  • 疾病相关Defects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
  • 序列相似性Contains 1 KBD domain.
    Contains 1 SPR (sprouty) domain.
    Contains 1 WH1 domain.
  • 翻译后修饰Phosphorylated on tyrosine.
  • 细胞定位Cell membrane. Membrane > caveola. Nucleus. Localized in cholesterol-rich membrane raft/caveola fractions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EVH1 domain-containing protein 1 antibody
    • EVH1/Sprouty domain containing protein antibody
    • FLJ33903 antibody
    • hSpred 1 antibody
    • hSpred1 antibody
    • NFLS antibody
    • PPP1R147 antibody
    • protein phosphatase 1 regulatory subunit 147 antibody
    • SPRE1_HUMAN antibody
    • SPRED 1 antibody
    • Spred-1 antibody
    • spred1 antibody
    • Sprouty related EVH1 domain containing 1 antibody
    • sprouty related EVH1 domain containing protein 1 antibody
    • Sprouty related protein 1 with EVH 1 domain antibody
    • Sprouty-related antibody
    • Suppressor of Ras/MAPK activation antibody
    see all

Anti-SPRED1 antibody 图像

  • Lane 1 : Anti-SPRED1 antibody (ab77079) at 1 µg/ml
    Lane 2 : Anti-SPRED1 antibody (ab77079) at 2 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • IHC image of ab77079 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77079, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab77079 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77079 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of Spred1 in Human Brain cells using ab77079 at 20 ug/ml.

Anti-SPRED1 antibody (ab77079)参考文献

This product has been referenced in:
  • Wang L  et al. miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. Int J Mol Sci 17:N/A (2016). WB ; Mouse . Read more (PubMed: 27338342) »
  • Ji JS  et al. Inhibition of microRNA-126 promotes the expression of Spred1 to inhibit angiogenesis in hepatocellular carcinoma after transcatheter arterial chemoembolization: in vivo study. Onco Targets Ther 9:4357-67 (2016). IHC-P . Read more (PubMed: 27499630) »

See all 3 Publications for this product

Product Wall

Thank you very much for your reply.
I'm sending a free of charge replacement vial of ab64740 on the order *** which should arrive tomorrow.
Please keep me updated about the performance of this antibody, and let me know if there is anything el...

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Thank you very much for completing our recent survey about your experience with us.

I am sorry the replacement SPRED1 antibody is also not working in WB. With ab64740, are you still observing the band ˜85 kDa, or are you not detecting any sig...

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Thank you very much for keeping me updated with the results.
I'm sorry to see that the suggestions were not more helpful. I'd be happy to send a different SPRED1 antibody to try. We also have ab64740 and ab62911-
http://www.abcam.com/SPRED1-a...

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Thank you for your reply. I am sorry the replacement is also not working in WB. With ab64740, are you still observing the band ˜85 kDa, or are you not detecting any signal? It would be very helpful if you could please provide an image of your results. ...

Read More

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of chargevial of ab64740 on the order ***, which should arrive tomorrow. Please keep me updated about th...

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Thank you very much for contacting us and letting us know about the trouble with this antibody.
To improve the results, I would suggest using 5% milk to block the membrane, and also blocking overnight at 4C. You an additionally load less than 40 u...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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Thank you very much for contacting us and letting us know about the trouble with this antibody.

The details and image you provided are very helpful. Could you also tell me how the samples were prepared prior to electrophoresis? How long were ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"