The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 67 kDa).
sppA protease IV is a serine type protease found in thylakoid membranes of photosynthetic organisms. The sppA protease family is represented in higher plants by one component, in most cyanobacteria – by two proteins (sppA1 and sppA2). sppA protease IV is a light induced protease and may be involved in the acclimation of photosynthetic membranes to high light.
Cell inner membrane; Multipass membrane protein
Endopeptidase IV antibody
Protease 4 homolog antibody
Protease IV antibody
Protease IV homolog antibody
Western blot - sppA protease IV antibody (ab65959)
Anti-sppA protease IV antibody (ab65959) at 1/2000 dilution + Synechocystis sp. PCC 6803 membrane proteins at 3 µg
Secondary Anti-rabbit at 1/30000 dilution Developed using the ECL technique