概述

  • 产品名称
  • 描述
    兔多克隆抗体to SPOP
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 326-375 of SPOP: INYHASDVLE TSGWKSMVVS HPHLVAEAYR SLASAQCPFL GPPRKRLKQS ,

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-SPOP antibody (ab81163) at 1 µg/ml + HepG2 lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/100000 dilution

    Predicted band size : 42 kDa

实验方案

文献

This product has been referenced in:
  • Gschweitl M  et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. Elife 5:e13841 (2016). Read more (PubMed: 27008177) »
  • Theurillat JP  et al. Prostate cancer. Ubiquitylome analysis identifies dysregulation of effector substrates in SPOP-mutant prostate cancer. Science 346:85-89 (2014). WB . Read more (PubMed: 25278611) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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