Recombinant fragment, corresponding to a region within amino acids 71-365 of Human SPOCK3 (AAH00460).
- 293T, A431, Jurkat and Raji cell lysates; U373 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol, 1% BSA
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Immunogen affinity purified
ab111897 is purified by antigen-affinity chromatography.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
SPOCK3 may participate in diverse steps of neurogenesis. It inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP and may also interfere with tumor invasion.
Secreted, extracellular space, extracellular matrix.
- HSAJ1454 antibody
- OTTHUMP00000219853 antibody
- OTTHUMP00000219854 antibody
Western blot - SPOCK3 antibody (ab111897)
Anti-SPOCK3 antibody (ab111897) at 1/5000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SPOCK3 antibody (ab111897)
ab111897 at 1/500 dilution staining SPOCK3 in Formalin-fixed, Paraffin-embedded U373 xenograft tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"