This antibody reacts specifically with human 56 kDa SPNS1 protein.
Synthetic peptide derived from N terminal region of human SPNS1 protein.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituents: Whole serum
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/500 - 1/2000. Predicted molecular weight: 56 kDa.
Sphingolipid transporter (By similarity). May be involved in necrotic or autophagic cell death.
Belongs to the major facilitator superfamily. Spinster family.
Mitochondrion inner membrane. Colocalizes with SDHB.
Information by UniProt
Western blot - Anti-SPNS1 antibody (ab59971)
All lanes : Anti-SPNS1 antibody (ab59971) at 1/1000 dilution Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa Additional bands at: 49 kDa (possible isoform)
Immunocytochemistry/ Immunofluorescence - Anti-SPNS1 antibody (ab59971)
ICC/IF image of ab59971 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59971, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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