Anti-splicing factor 1抗体(ab58077)

概述

  • 产品名称Anti-splicing factor 1抗体
    参阅全部 splicing factor 1 一抗
  • 描述
    小鼠单克隆抗体to splicing factor 1
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-111 of Human splicing factor 1

性能

应用

Our Abpromise guarantee covers the use of ab58077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.

靶标

  • 相关性SF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • BBP antibody
    • D11S636 antibody
    • Mammalian branch point binding protein mBBP antibody
    • OTTHUMP00000069749 antibody
    • OTTHUMP00000069750 antibody
    • OTTHUMP00000069752 antibody
    • Splicing factor 1 antibody
    • Transcription factor ZFM1 antibody
    • ZFM1 antibody
    • Zinc finger gene in MEN1 locus antibody
    • Zinc finger protein 162 antibody
    • ZNF162 antibody
    see all

Anti-splicing factor 1 antibody 图像

  • Immunofluorescence of  HeLa cells using ab58077 at 10 μg/ml.

  • Immunoprecipitation of SF1 transfected lysate using anti-SF1 monoclonal antibody ab58077 and Protein A Magnetic Bead and immunoblotted with SF1 monoclonal antibody ab58077

  • splicing factor 1 antibody (ab58077) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon.
  • Western blot against tagged recombinant protein immunogen using ab58077 splicing factor 1 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

Anti-splicing factor 1 antibody (ab58077)参考文献

ab58077 has not yet been referenced specifically in any publications.

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