The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
Use a concentration of 5 µg/ml.
Use a concentration of 10 µg/ml.
Use at an assay dependent concentration.
相关性SF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.