概述

  • 产品名称Anti-SPINK1抗体
    参阅全部 SPINK1 一抗
  • 描述
    兔多克隆抗体to SPINK1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 10 to the C-terminus of Human SPINK1.

  • 阳性对照
    • This antibody gave a positive signal in Human Pancreas tissue lysate within both Western blot and Immunohistochemistry.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
    In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
  • 疾病相关Pancreatitis, hereditary
    Tropical calcific pancreatitis
  • 序列相似性Contains 1 Kazal-like domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ISK1_HUMAN antibody
    • Pancreatic secretory trypsin inhibitor antibody
    • Pancreatic serine protease inhibitor Kazal antibody
    • PCTT antibody
    • PSTI antibody
    • Serine protease inhibitor Kazal type 1 antibody
    • Serine protease inhibitor Kazal-type 1 antibody
    • Spink 3 antibody
    • SPINK1 antibody
    • Spink3 antibody
    • TaTI antibody
    • TCP antibody
    • Tumor associated trypsin inhibitor antibody
    • Tumor-associated trypsin inhibitor antibody
    see all

Anti-SPINK1 antibody 图像

  • IHC image of SPINK1 staining in humn pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab98183, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Anti-SPINK1 antibody (ab98183) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 8 kDa
    Observed band size : 8 kDa
    Additional bands at : 60 kDa,70 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-SPINK1 antibody (ab98183)参考文献

ab98183 has not yet been referenced specifically in any publications.

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