概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to SPINK1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: DSLGREAKCY NELNGCTKIY DPVCGTDGNT YPNECVLCFE NRKRQTSILI QKSGPC, corresponding to amino acids 24-80 of Human SPINK1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab183347 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab58227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 1 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
      In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
    • 疾病相关
      Pancreatitis, hereditary
      Tropical calcific pancreatitis
    • 序列相似性
      Contains 1 Kazal-like domain.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ISK1_HUMAN antibody
      • Pancreatic secretory trypsin inhibitor antibody
      • Pancreatic serine protease inhibitor Kazal antibody
      • PCTT antibody
      • PSTI antibody
      • Serine protease inhibitor Kazal type 1 antibody
      • Serine protease inhibitor Kazal-type 1 antibody
      • Spink 3 antibody
      • SPINK1 antibody
      • Spink3 antibody
      • TaTI antibody
      • TCP antibody
      • Tumor associated trypsin inhibitor antibody
      • Tumor-associated trypsin inhibitor antibody
      see all

    图片

    • SPINK1 antibody (ab58227) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human pancreas.


    • Observed band size : 29 kDa (why is the actual band size different from the predicted?)
      Western blot against tagged recombinant protein immunogen using ab58227 SPINK1 antibody at 1ug/ml. Predicted band size of immunogen is 29 kDa

    文献

    This product has been referenced in:
    • Lotan TL  et al. Somatic molecular subtyping of prostate tumors from HOXB13 G84E carriers. Oncotarget 8:22772-22782 (2017). IHC ; Human . Read more (PubMed: 28186998) »
    • Noh BJ  et al. Prognostic value of ERG, PTEN, CRISP3 and SPINK1 in predicting biochemical recurrence in prostate cancer. Oncol Lett 11:3621-3630 (2016). IHC . Read more (PubMed: 27284364) »

    See all 5 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab58227.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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