概述

  • 产品名称Anti-Spindly抗体
    参阅全部 Spindly 一抗
  • 描述
    兔多克隆抗体to Spindly
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 475 and 525 of Human CCDC99 (NP_060255.2).

  • 阳性对照
    • HeLa whole cell lysate or 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab99344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
  • 序列相似性Belongs to the Spindly family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA409762 antibody
    • Arsenite related gene 1 protein antibody
    • Arsenite-related gene 1 protein antibody
    • CCDC 99 antibody
    • Ccdc99 antibody
    • Coiled coil domain containing 99 antibody
    • Coiled-coil domain-containing protein 99 antibody
    • FLJ20364 antibody
    • FLJ40690 antibody
    • hSpindly antibody
    • MGC124977 antibody
    • Protein Spindly antibody
    • rhabdomyosarcoma antigen MU RMS 40.4A antibody
    • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
    • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
    • SPDL1 antibody
    • SPDLY_HUMAN antibody
    • Spindle apparatus coiled coil domain containing protein 1 antibody
    • Spindle apparatus coiled coil protein 1 antibody
    • Spindly antibody
    • Spindly homolog antibody
    see all

Anti-Spindly antibody 图像

  • All lanes : Anti-Spindly antibody (ab99344) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 70 kDa


    Exposure time : 3 minutes
  • 3µg ab99344 were used to precipitate CCDC99 from 1mg HeLa whole cell lysate.
    20% IP was loaded on gel and probed with ab99344 at 1µg/ml.
    Detection: chemiluminescence with exposure time of 10 seconds

Anti-Spindly antibody (ab99344)参考文献

ab99344 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99344.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"