概述

  • 产品名称Anti-Spindly抗体
    参阅全部 Spindly 一抗
  • 描述
    小鼠单克隆抗体to Spindly
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant full length Human CCDC99 protein (AAH12568, 1 a.a. ~ 606 a.a) with tag.

  • 阳性对照
    • Recombinant tagged full length human CCDC99.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
    • 序列相似性Belongs to the Spindly family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AA409762 antibody
      • Arsenite related gene 1 protein antibody
      • Arsenite-related gene 1 protein antibody
      • CCDC 99 antibody
      • Ccdc99 antibody
      • Coiled coil domain containing 99 antibody
      • Coiled-coil domain-containing protein 99 antibody
      • FLJ20364 antibody
      • FLJ40690 antibody
      • hSpindly antibody
      • MGC124977 antibody
      • Protein Spindly antibody
      • rhabdomyosarcoma antigen MU RMS 40.4A antibody
      • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
      • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
      • SPDL1 antibody
      • SPDLY_HUMAN antibody
      • Spindle apparatus coiled coil domain containing protein 1 antibody
      • Spindle apparatus coiled coil protein 1 antibody
      • Spindly antibody
      • Spindly homolog antibody
      see all

    Anti-Spindly antibody 图像

    • Anti-Spindly antibody (ab76670) at 1 µg/ml + Recombinant tagged full length human CCDC99 at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 70 kDa
      Observed band size : 93 kDa (why is the actual band size different from the predicted?)

    Anti-Spindly antibody (ab76670)参考文献

    ab76670 has not yet been referenced specifically in any publications.

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