Western blot - Anti-spindlin 1 antibody (ab118784)
Anti-spindlin 1 antibody (ab118784) at 1 µg/ml + Human fetal brain lysate at 10 µg
Predicted band size : 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-spindlin 1 antibody (ab118784)Image courtesy of Peter Zentis by Abreview.
ab118784 staining spindlin 1 in human MDA-MB-468 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA for 15 minutes at 37°C and then incubated with ab118784 at a 1/100 dilution for 1 hour at 37°C. The secondary used was an ATTO488 conjugated F(ab')2 fragment donkey anti-rabbit polyclonal used at a 1/50 dilution.
Ducroux A et al. The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1. PLoS Pathog10:e1004343 (2014).
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