概述

  • 产品名称Anti-SPIN3抗体
  • 描述
    兔多克隆抗体to SPIN3
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 31-161 of Human SPIN3 (Q5JUX0).

  • 阳性对照
    • Human bone marrow tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

靶标

Anti-SPIN3 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab121115 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121115 at 1/150 dilution staining SPIN3 in Paraffin-emebedded Human bone marrow tissue by Immunohistochemistry. Note: strong nuclear and cytoplasmic positivity in bone marrow poietic cells.

Anti-SPIN3 antibody (ab121115)参考文献

ab121115 has not yet been referenced specifically in any publications.

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