概述

  • 产品名称Anti-SPHK2抗体
    参阅全部 SPHK2 一抗
  • 描述
    兔多克隆抗体to SPHK2
  • 经测试应用适用于: IP, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, derived from within residues 600-700 of SPHK2 (Human).

  • 阳性对照
    • Human cancer tissue, transfected 293 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab37977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 24659784
ELISA 1/1000.
WB 1/1000. Predicted molecular weight: 69 kDa.
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

靶标

Anti-SPHK2 antibody 图像

  • Anti-SPHK2 antibody (ab37977) at 1/1000 dilution + HepG2 cell Lysate at 35 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size : 69 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical staining of SPHK2 in Human breast carcinoma tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab37977 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-SPHK2 antibody (ab37977) at 1/500 dilution + Mouse NIH 3T3 whole cell lysate at 25 µg

    Secondary
    An HRP-conjugated Donkey Polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

Anti-SPHK2 antibody (ab37977)参考文献

This product has been referenced in:
  • Testai FD  et al. Changes in the metabolism of sphingolipids after subarachnoid hemorrhage. J Neurosci Res 93:796-805 (2015). WB ; Human . Read more (PubMed: 25597763) »
  • Panneer Selvam S  et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation. Sci Signal 8:ra58 (2015). Read more (PubMed: 26082434) »

See all 8 Publications for this product

Product Wall

Thank you for your email.

Yes, I meant His or GST tag etc.

It is more likely that the band appeared in SPHK2 blot is the specific one. However it can be further testified by
- trying no primary control.
- blocking the antibody...

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Thank you for your email.

It is true that the emails are redirected to us if you reply to mailto:technical@abcam.com. I would advise you to log in into your account and check the CCE thread this is the easiest way for tracking the conversation...

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Thank you for your email.

I am sorry I might have missed the information about LDS buffer as cell lysis buffer - indeed there are companies who sells these for cell lysis.

I have requested specific protocol from laboratory I will send...

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Thank you for contacting us.

Just to confirm I have started working on your inquiry so I will get back to you with more information soon.

There is one comment to make here;

ab37977; This SPHK2 antibody is generated from rabb...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Le numéro de commande de remplacement gratuit avec la référenc...

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Thank you for your call earlier this week and for your patience. The blocking peptide of ab37977 is now available on the catalog as ab115809- http://www.abcam.com/SPHK2-peptide-ab115809.html Please let me know if you have any quest...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount 25 µg
Specification NIH 3T3
Treatment hypoxia
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HMEC endothelial cells)
Loading amount 25 µg
Specification HMEC endothelial cells
Treatment hypoxia
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"