概述

  • 产品名称
  • 描述
    兔多克隆抗体to SPHK1
  • 特异性
    ab46719 reacts with Human SPHK 1 and Murine SPHK 1.
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Cynomolgus monkey, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    DLESEKYRRLG

    , corresponding to amino acids 264-274 of Human SPHK1

  • 阳性对照
    • Human platelet microsomes, Murine kidney, Murine spleen and Raji cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab46719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/200. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/80.

靶标

图片

  • All lanes : Anti-SPHK1 antibody (ab46719) at 2 µg/ml

    Lane 1 : Human platelet microsomes (20ug)
    Lane 2 : Murine kidney pellet (20ug)
    Lane 3 : Murine spleen supernatant( 40ug)
    Lane 4 : Raji cell lysate (40ug)


    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • King A  et al. Sphingosine-1-Phosphate Prevents Egress of Hematopoietic Stem Cells From Liver to Reduce Fibrosis. Gastroenterology 153:233-248.e16 (2017). WB ; Mouse . Read more (PubMed: 28363640) »
  • Hirata N  et al. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation. Nat Commun 5:4806 (2014). Read more (PubMed: 25254944) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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