Anti-SPHK1抗体(ab228645)
Key features and details
- Rabbit polyclonal to SPHK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SPHK1抗体
参阅全部 SPHK1 一抗 -
描述
兔多克隆抗体to SPHK1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human SPHK1 (internal sequence). The exact sequence is proprietary.
Database link: Q9NYA1 -
阳性对照
- WB: MOLT-4 whole cell lysate. ICC/IF: A-375 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab228645于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. -
组织特异性
Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. -
序列相似性
Contains 1 DAGKc domain. - Information by UniProt
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数据库链接
- Entrez Gene: 8877 Human
- Omim: 603730 Human
- SwissProt: Q9NYA1 Human
- Unigene: 68061 Human
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别名
- SK 1 antibody
- SK1 antibody
- Sphingosine kinase 1 antibody
see all
图片
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Anti-SPHK1 antibody (ab228645) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 43 kDa10% SDS-PAGE gel.
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4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for SPHK1 (green) using ab228645 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab228645 尚未被引用在任何文献中。