Anti-Sphingomyelin Synthase 2抗体(ab87214)

概述

  • 产品名称Anti-Sphingomyelin Synthase 2抗体
    参阅全部 Sphingomyelin Synthase 2 一抗
  • 描述
    兔多克隆抗体to Sphingomyelin Synthase 2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72 - 121 (

    FPLEWWKTGIAFIYAVFNLVLTTVMITVVHERVPPKELSPPLPDKFFDYI

    ) of Human Sphingomyelin Synthase 2 (NP_689834)

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab87214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Does not function strictly as a SM synthase. Required for cell growth.
  • 组织特异性Brain, heart, kidney, liver, muscle and stomach.
  • 序列相似性Belongs to the sphingomyelin synthase family.
  • 翻译后修饰Palmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization.
  • 细胞定位Cell membrane. Golgi apparatus membrane. Predominantly plasma membrane, some in Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC26963 antibody
    • Phosphatidylcholine:ceramide cholinephosphotransferase 2 antibody
    • SGMS 2 antibody
    • Sgms2 antibody
    • SM synthase antibody
    • SMS 2 antibody
    • SMS2 antibody
    • SMS2_HUMAN antibody
    • Sphingomyelin synthase 2 antibody
    see all

Anti-Sphingomyelin Synthase 2 antibody 图像

  • Anti-Sphingomyelin Synthase 2 antibody (ab87214) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

Anti-Sphingomyelin Synthase 2 antibody (ab87214)参考文献

This product has been referenced in:
  • Yachi R  et al. Subcellular localization of sphingomyelin revealed by two toxin-based probes in mammalian cells. Genes Cells 17:720-7 (2012). WB ; African Green Monkey . Read more (PubMed: 22747662) »

See 1 Publication for this product

Product Wall

The difference is between the immunogens. Both were generated against 14aa length peptides from the region of 72 - 121aa but the peptides differ. Again apologies for the long wait.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"