Anti-Sphingomyelin Synthase 1抗体(ab235057)
Key features and details
- Rabbit polyclonal to Sphingomyelin Synthase 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Sphingomyelin Synthase 1抗体
参阅全部 Sphingomyelin Synthase 1 一抗 -
描述
兔多克隆抗体to Sphingomyelin Synthase 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Pig -
免疫原
Recombinant fragment corresponding to Human Sphingomyelin Synthase 1 aa 1-150.
Database link: Q86VZ5 -
阳性对照
- WB: HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Human pancreatic tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
>95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab235057于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/5000. Predicted molecular weight: 49 kDa.
|
|
IHC-P |
1/200 - 1/500.
|
说明 |
---|
WB
1/500 - 1/5000. Predicted molecular weight: 49 kDa. |
IHC-P
1/200 - 1/500. |
靶标
-
相关性
Sphingomyelin synthase 1 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Sphingomyelin synthase 1 is required for cell growth and is also capable of suppressing BAX-mediated apoptosis and preventing cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. It may protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide. -
细胞定位
Golgi Apparatus. Multi-pass membrane protein -
数据库链接
- Entrez Gene: 259230 Human
- Entrez Gene: 208449 Mouse
- Entrez Gene: 353229 Rat
- Omim: 611573 Human
- SwissProt: Q86VZ5 Human
- SwissProt: Q8VCQ6 Mouse
- SwissProt: Q7TSX5 Rat
-
别名
- MOB antibody
- Phosphatidylcholine:ceramide cholinephosphotransferase 1 antibody
- Protein Mob antibody
see all
图片
-
All lanes : Anti-Sphingomyelin Synthase 1 antibody (ab235057) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa -
Paraffin-embedded human pancreatic tissue stained for Sphingomyelin Synthase 1 using ab235057 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab235057 尚未被引用在任何文献中。