Anti-Spermine抗体(ab6466)

概述

  • 产品名称Anti-Spermine抗体
    参阅全部 Spermine 一抗
  • 描述
    兔多克隆抗体to Spermine
  • 特异性Using a conjugate Spermine-Glutaric Anhydride-Protein, antibody specificity was performed with a peptide ELISA test by competition experiments with the following compounds:

    Compound

    Cross-reactivity ratio (a)

    Spermine-GA-BSA

    1

    Spermidine-GA-BSA

    1/3

    Diamino-Pentane-G-BSA

    1/700

    Ornithine-GA-BSA

    1/500

    (a) Spermine-GA-BSA concentration/unconjugated or conjugated polyamine concentration at half displacement

    G = Glutaraldehyde, GA = Glutaric Anhydride, BSA = Bovine Serum Albumin

  • 经测试应用适用于: IHC-FoFrmore details
    不适用于: ELISA
  • 免疫原

    Chemical/ Small Molecule.

性能

应用

Our Abpromise guarantee covers the use of ab6466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr
  • 应用说明IHC-FoFr: 1/2000 - 1/5000. Please note the fixative must be a multistep fixation with MES/ECD/paraformaldehyde. See recommended protocol and fixative details below.

    Is unsuitable for whole cell ELISA (only suitable for peptide ELISA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Spermine, a polyamine, is a positively charged compound found in living cells and can cause conformational changes in DNA via charge and solubility mechanisms which can affect gene expression cell differentiation. Spermine may be linked to the development of cancer.
    • 别名
      • Spermine antibody

    Anti-Spermine antibody (ab6466)参考文献

    ab6466 has not yet been referenced specifically in any publications.

    Product Wall

    According to our records, ab6466 was proving difficult to use inIHC and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand ...

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    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. As we discussed on the phone, ...

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    Thank you very much for your interest in ab6466. To our knowledge, this antibody has not been tested in immunoprecipitation. However, I can offer you a discount off a future purchase if you purchase ab6466 now, test it in immunoprecipitation and su...

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    I have been informed that this antibody has only been tested in peptide ELISA and we do not think it will be suitable for whole cell ELISA. The sensitivity of the antibody in peptide ELISA is detailed in the specificity section on the datasheet, ple...

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    ELISA protocol for the anti-conjugated Spermine antibody (rabbit) detection and possible characterization Coating on microtitration plates (Merckeurolab). 10 microgrammes of conjugated Spermine-AG-BSA per ml of carbonate buffer (pH = 9,6). Take this...

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    All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). We will update the on-line datasheet of this product as soon as get more data. We produce some of ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"