概述

  • 产品名称
    Spermidine hydrochloride
  • 描述
    Endogenous polyamine. nNOS inhibitor.
  • 生物学描述
    Endogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
  • 纯度
    > 98%

性能

应用

Our Abpromise guarantee covers the use of ab120057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
    The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us.

- The datasheets can be downloaded from the links;

http://www.abcam.com/Spermine-ab120051.html

http://www.abcam.com/Spermidine-hydrochloride-ab120057.html

- These products are for researc...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

注册