概述

  • 产品名称Anti-Spermidine抗体
  • 描述
    兔多克隆抗体to Spermidine
  • 特异性Reacts with spermidine and cross reacts with spermine.
  • 经测试应用适用于: IHC-P, WBmore details
  • 免疫原

    Spermidine conjugated to KLH.

性能

应用

Our Abpromise guarantee covers the use of ab7318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
  • 应用说明IHC-P: Use at an assay dependent dilution (PMID 19158080).
    WB: 1/200 - 1/5000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Spermidine is a polyamine compound was originally isolated from semen. It is found ribosomes and living tissues and has various metabolic functions.

    Anti-Spermidine antibody 图像

    • ab7318 staining Spermidine in rat lungs by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent fixation in 4% paraformaldehyde overnight and embedded in paraffin, heat mediate antigen retrieval was performed in target retrieval solution and sections were immersed in 3% hydrogen peroxide for 5 min to deplete the endogenous peroxidase activity. Sections were incubated in 1% bovine serum albumin in PBS for 30 min followed by primary antibody at room temperature for 2 h. A primary antibody Enhancer and HRP polymer were used according to the manufacturer's instructions. Left image show staining with ab7318 while the right one show staining with same procedure without primary antibody.

    Anti-Spermidine antibody (ab7318)参考文献

    This product has been referenced in:
    • Liao CP  et al. Pneumocystis mediates overexpression of antizyme inhibitor resulting in increased polyamine levels and apoptosis in alveolar macrophages. J Biol Chem 284:8174-84 (2009). IHC-P . Read more (PubMed: 19158080) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    Spermidine and spermine are both polyamines found in living tissues and have various metabolic functions. As they are not proteins, they are not species specific. If the tissue you are interested in probing has s...

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    Thank you for contacting us and your interest in our products.

    The two antibodies which you are interested in (ab7318 and ab26975) are both formulations of the whole antiserum. This means that quantification of the exact concentration is not...

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    I just wanted to let you know that I am still working on your enquiry. I am still in contact with the originator of this antibody for the information you requested, and am awaiting for an acceptable reply. The originator assures me that there will be...

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    This antibody reacts with spermidine molecule and there is no differences between spermidine of different species. This antibody cannot be use to identify spermidine synthase.

    Thank you for your enquiry. As mentioned previously by my colleagues; unfortunately I cannot provide you with further information as this antibody is sourced. The originator of this antibody has not provided details of how the western blot was perf...

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    This antibody does not cross react with putrescine but it cross reacts with spermine (1:1). We unfortunately do not have any references to published articles using this antibody. The epitope has not been mapped but as the molecule is very short we can'...

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    This antibody does not cross react with putrescine but it cross reacts with spermine (1:1). We unfortunately do not have any references to published articles using this antibody. The epitope has not been mapped but as the molecule is very short we can'...

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    Thank you for your email. I have contacted the originator of this antibody to see if they can provide any additional information, and at this time, I am unable to obtain anything further to what we already have on the online datasheet. We will update t...

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    Thank you for your email. This particular antibody was originated outside Abcam and we don't have any additional additional information other than what is contained on the online datasheet. This antibody was not characterized and tested in-house, but b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"