antigen, corresponding to amino acids 1-81 (MKKVKKKRSE ARRHRDSTSQ HASSNSTSQQ PSPESTPQQP SPESTPQHSS LETTSRQPAF QALPAPEIRR SSCCLLSPDA N) of Human SPATA3.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SPATA3 antibody (ab121566)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab121566 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA3 antibody (ab121566)
ab121566 at 1/150 dilution staining SPATA3 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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