概述

  • 产品名称
    Anti-SPATA20抗体
    参阅全部 SPATA20 一抗
  • 描述
    兔多克隆抗体to SPATA20
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 647-756 of Human SPATA20 (Q8TB22).

  • 阳性对照
    • Human testis tissue. RT-4 and U-251 MG cell lysate. Human plasma lysate. Human liver and tonsil tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab121918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

图片

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus. Recommended concentration of ab121918 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121918 at 1/50 dilution staining SPATA20 in paraffin-embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-SPATA20 antibody (ab121918) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

文献

ab121918 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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